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Inhibitory action of DIDS on chloride transport across the amphibian cornea.
Author(s) -
Bentley P J,
McGahan M C
Publication year - 1980
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1980.sp013340
Subject(s) - dids , bumetanide , cornea , chemistry , toad , biophysics , inhibitory postsynaptic potential , chloride , aqueous solution , furosemide , ion transporter , biochemistry , endocrinology , membrane , biology , ophthalmology , organic chemistry , medicine
1. DIDS (4,4'‐diisothiocyano‐2,2'‐stilbene disulphonic acid) reduced the CI Isc across the toad's (Bufo marinus) cornea. It acted on either the aqueous or tear side and these effects were additive. 2. The reduction in the Isc was equivalent to the decline in the undirectional flux of Cl from the aqueous to the tear side. The Cl flux from tear to aqueous was not changed. 3. DIDS did not change transmural Na transport. 4. The action of the diuretic bumetanide, which also inhibits Cl transport was additive to that of DIDS when both compounds were present on the aqueous though not when they were on the tear side of the cornea. 5. The results are consistent with the role of a Cl‐/anion exchange mechanism in active Cl transport across the cornea. 6. A hypothesis regarding the interactions and site of action of bumetanide in relation to that of DIDS, and the Cl transport process, is proposed.