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A method for investigating the role of calcium in the shape change, aggregation and serotonin release of rat platelets.
Author(s) -
Belville J S,
Bennett W F,
Lynch G
Publication year - 1979
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1979.sp013040
Subject(s) - calcium , thrombin , platelet , chemistry , serotonin , biophysics , platelet aggregation , medicine , extracellular , endocrinology , creatine , concanavalin a , biochemistry , receptor , biology , in vitro , organic chemistry
1. Effects of the platelet secretagogues thrombin and Concanavalin A (Con A) on shape change and release have been studied under conditions where external Ca2+ and ADP have been controlled. 2. In the absence of detectable aggregation both Con A‐ and thrombin‐induced release of serotonin are markedly dependent upon extracellular calcium. 3. Con A, unlike thrombin, does not produce aggregation in calcium‐supplemented medium. 4. The above property of Con A suggests that, in combination with added creatine phosphate and creatine phosphokinase, this ligand will be of value in the analysis of calcium‐dependent release in platelets.