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T‐tubule swelling in hypertonic solutions: a freeze substitution study.
Author(s) -
Franzini-Armstrong C,
Heuser J E,
Reese T S,
Somlyo A P,
Somlyo A V
Publication year - 1978
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1978.sp012492
Subject(s) - tonicity , endoplasmic reticulum , swelling , biophysics , chemistry , sodium , electron microscope , rana , anatomy , sucrose , biochemistry , biology , medicine , pathology , physics , organic chemistry , optics
Striated muscles from Rana pipiens have been exposed for variable periods of time to Ringer solutions made hypertonic by addition of either sucrose or sodium chloride. The muscles have been rapid‐frozen and then prepared for electron microscopy by either freeze‐substitution, freeze‐fracture or cryoultramicrotomy. The only compartment greatly affected by hypertonicity is the transverse tubular system, which is visibly swollen. None of the elements of the sarcoplasmic reticulum increase in size.