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Competitive antagonism by phentolamine of responses to biogenic amines and the transmitter at a neuroglandular junction.
Author(s) -
Bowser-Riley F,
House C R,
Smith R K
Publication year - 1978
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1978.sp012357
Subject(s) - phentolamine , antagonism , neurotransmitter , chemistry , endocrinology , medicine , stimulation , dopamine , antagonist , receptor , competitive antagonist , pharmacology , biology , biochemistry
1. A quantitative study has been made of phentolamine's inhibition of the electrical and secretory responses of the isolated salivary glands of Nauphoeta cinerea Olivier to nerve stimulation and bath applications of agonists. 2. The results suggested that phentolamine is a competitive antagonist having an affinity constant of about 1 micrometer‐1 for the receptors for dopamine, noradrenaline, adrenaline and the neurotransmitter. 3. Phentolamine also inhibited responses to 5‐hydroxytryptamine in a manner seemingly more complex than competitive antagonism. Attempts to estimate the affinity constant gave values of about 0.08 and 0.015 microgram‐1 for inhibition of the secretory and electrical responses respectively. 4. This investigation showed that phentolamine discriminates between two kinds of receptor in this gland, one binding 5‐hydroxytryptamine and the other combining with catecholamines and the neurotransmitter.