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The effects of pH and curare on the time course of end‐plate currents at the neuromuscular junction of the frog.
Author(s) -
Mallart A,
Molgó J
Publication year - 1978
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1978.sp012238
Subject(s) - chemistry , curare , cholinesterase , neuromuscular junction , biophysics , acetylcholine , time constant , stimulation , motor nerve , membrane potential , anesthesia , biochemistry , anatomy , endocrinology , neuroscience , electrical engineering , biology , medicine , engineering
1. The effect of pH changes on synaptic currents has been analysed by external recording of the miniature end‐plate currents (m.e.p.c.s) or by recording in voltage‐clamped end‐plates the current elicited by nerve stimulation (e.p.c.). 2. Changes in pH do not appreciably effect the peak amplitude of the current produced by a single quantum or by short ionophoretic pulses of acetylcholine. 3. The time constant of decay of the m.e.p.c.s is prolonged by about 50% in acid pH and shortened by about the same amount in alkaline pH. This effect is independent of the cholinesterase activity of the end‐plate. 4. In curarized preparations the decay of the e.p.c. is shorter than in Mg‐blocked end‐plate even in the absence of cholinesterase blocking agents. 5. The action of pH on the decays can be explained by a titration of the surface charges of the membrane which effects the voltage dependent reaction that controls the rate of closing of the synaptic channels.