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Movement of strontium ions into mast cells and its relationship to the secretory response
Author(s) -
Foreman J. C.,
Hallett M. B.,
Mongar J. L.
Publication year - 1977
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1977.sp011998
Subject(s) - histamine , secretion , chemistry , calcium , mole , metabolism , endocrinology , medicine , incubation , mast cell , biology , biochemistry , immunology , organic chemistry
1. Non‐stimulated mast cells take up 89 Sr and 45 Ca. There is a rapid phase of uptake of both labels which is complete in 1 min and the 89 Sr uptake is similar in magnitude to the 45 Ca uptake. A further slower phase of uptake occurs in the period from 1 to 30 min of incubation. The magnitude of this slower phase is about 6 times greater for 89 Sr than for 45 Ca. 2. Non‐stimulated and antigen‐stimulated mast cells accumulate Sr which can be measured by atomic absorption spectroscopy. There is a direct relationship between Sr accumulation and histamine secretion which is independent of whether or not the cells are stimulated. 10% histamine secretion is associated with Sr accumulation of 0.25 f‐mole/cell. 3. The time course of 89 Sr uptake is similar to the time course of histamine secretion in non‐stimulated cells. 4. The uptake of 89 Sr is linearly related to the external Sr concentration in the range 0.5‐16 m‐mole/l. for both stimulated and non‐stimulated cells. 5. Ca, 10‐1000 μmole/l. inhibits 89 Sr uptake in non‐stimulated cells. 6. Inhibition of glycolysis and oxidative phosphorylation prevents spontaneous histamine secretion in the presence of Sr without blocking the accumulation of Sr by the cells. 7. Dibutyryl cyclic AMP, 10 m‐mole/l. produces only 18% inhibition of 89 Sr uptake whereas histamine secretion is inhibited by 45% by the same concentration. The antigen‐stimulated Sr uptake on the other hand can be completely inhibited by dibutyryl cyclic AMP, 10 m‐mole/l. 8. The uptakes of 89 Sr and Sr by unstimulated mast cells after 60 min incubation are dependent on extracellular H ion concentration. Both uptakes increase with increasing pH over the range pH 7‐8.5.