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Regulation of amylase release from dispersed pancreatic acinar cells.
Author(s) -
Gardner J D,
Jackson M J
Publication year - 1977
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1977.sp011961
Subject(s) - secretin , medicine , vasoactive intestinal peptide , endocrinology , amylase , cholecystokinin , chemistry , adenosine , cholinergic , cyclic guanosine monophosphate , acinus , cyclic adenosine monophosphate , guanosine , neuropeptide , biology , pancreas , enzyme , biochemistry , receptor , nitric oxide
1. A study has been made of factors influencing release of amylase from dispersed pancreatic acinar cells. 2. In the basal, unstimulated, condition cells released 2‐3% of the total amylase present in 30 min. 3. The rate of amylase release was stimulated 50‐70% by C‐terminal octapeptide of cholecystokinin (CCK‐OP, maximally effective concentration, 3 X 10(‐10) M); carbamylcholine (maximally effective concentration, 10(‐5 M); secretin (maximally effective concentration greater than 10(‐6) M); vasoactive intestinal peptide (VIP, maximally effective concentration, 10(‐8) M); and adenosine 3':5' monophosphate (cyclic AMP) and guanosine 3':5' monophosphate (cyclic GMP) as well as their dibutyryl derivatives (maximally effective concentrations, 10(‐3) M). 4. The responses to CCK‐OP or carbamylcholine were potentiated by secretin, VIP or dibutyryl cyclic AMP. 5. The responses to secretin or VIP were potentiated by CCK‐OP, carbamylcholine, or dibutyryl cyclic GMP. 6. There appear to be two pathways for the regulation of amylase release from pancreatic acinar cells: one pathway can be stimulated by cholecystokinin or cholinergic agonists, and the response to these stimuli is mediated by cyclic GMP; the other pathway can be stimulated by secretin or VIP, and the response to these stimuli is mediated by cyclic AMP.