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The concentration of ionized magnesium in barnacle muscle fibres.
Author(s) -
Brinley F J,
Scarpa A,
Tiffert T
Publication year - 1977
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1977.sp011781
Subject(s) - mole , chemistry , magnesium , intracellular , phosphate , dialysis , chromatography , bound water , analytical chemistry (journal) , nuclear chemistry , biochemistry , medicine , organic chemistry , molecule
1. The total Mg in isolated fibres of Balanus aquila was 10‐5 m‐mole/kg wet wt. 2. The intracellular free Mg was measured by a null point method using Eriochrome Blue as an indicator of free Mg, and internal dialysis with solutions of varying ionized Mg concentrations. The results indicated a free Mg of 6 mM or 4‐2 m‐mole/kg wet wt. in the intracellular water immediately surrounding the dialysis capillary. 3. The ATP concentration was estimated to be 4‐9 m‐mole/kg wet wt. 4. A tentative partitioning of Mg among various intracellular constitutents based on present data combined with published work by others is (m‐mole/kg wet wt): free, 4‐2; MgATP, 4‐2; myofibrillar bound, 1; residual (presumably bound to arginine phosphate and phosphate) ca. 1.