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Metabolic energy dependence of glucose, water and sodium absorption in the presence and absence of 'downhill' sodium gradients across isolated rat small intestine.
Author(s) -
Gardner M L
Publication year - 1977
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1977.sp011714
Subject(s) - sodium , chemistry , absorption (acoustics) , medicine , small intestine , metabolism , concentration gradient , endocrinology , biophysics , biochemistry , chromatography , biology , materials science , organic chemistry , composite material
1. Absorption of glucose and water was studied in isolated perfused rat small intestine with arterial infusion under normal conditions and with a 'downhill' sodium gradient across the mucosa. 2. The effects of metabolic inhibitors, iodoacetate phenformin and dinitrophenol, on absorption were determined in the presence of normal and 'downhill' sodium gradients. 3. Glucose absorption was inhibited by each inhibitor, and the inhibition was independent of the direction of the sodium gradient. 4. Net sodium flux across the mucosa was also inhibited under 'downhill' sodium gradient conditions. 5. The simultaneous and parallel inhibition of both glucose and sodium transport is consistent with, but does not prove, the sodium gradient theory.