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The preparation of human red cell ghosts containing calcium buffers.
Author(s) -
Simons T J
Publication year - 1976
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1976.sp011321
Subject(s) - intracellular , calcium , biophysics , chelation , haemolysis , chemistry , permeability (electromagnetism) , cell permeability , biochemistry , nuclear chemistry , membrane , inorganic chemistry , biology , organic chemistry , immunology
1. Ca buffers may be introduced into human red cells by reversible haemolysis. The resealed ghosts retain Ca and chelating anions in the same ratio as in the haemolysing solution, enabling the intracellular Ca2+ concentration to be calculated simply. 2. The passive permeability of the ghosts to Na and Cl is unaffected by intracellular Ca2+ concentrations in the 10(‐8)‐10(‐4) M range, whereas the K permeability is greatly increased at concentrations above 10(‐7) M. 3. These preparations enable Ca‐dependent K movements to be studied under stable conditions. When the ghosts contain about 5 X 10(‐6) M‐Ca2+, over 96% of K transport occurs via the Ca‐sensitive route.