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Cellular specificity of serotonin storage and axonal transport in identified neurones of Aplysia californica
Author(s) -
Goldman James E.,
Schwartz James H.
Publication year - 1974
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1974.sp010694
Subject(s) - aplysia , serotonin , neuroscience , chemistry , axoplasmic transport , biology , biophysics , biochemistry , receptor
1. [ 3 H] D,L ‐5‐hydroxytryptophan ([ 3 H]5HTP) was injected under pressure into cell bodies of identified cholinergic and serotonergic neurones in the central nervous system of the marine mollusc, Aplysia californica . 2. Both serotonergic and cholinergic neurones converted [ 3 H]5HTP to [ 3 H]5‐hydroxytryptamine ([ 3 H]5HT). 3. The fate of [ 3 H]5HT in the two types of neurones differed. In serotonergic cells, 5HT was present primarily in particulate form; the transmitter readily moved from cell bodies into nerves by selective transport. In contrast, 5HT remained free in the cytoplasm of the cholinergic neurone, and was not transported from the cell body. 4. Treatment of Aplysia with reserpine decreased the proportion of [ 3 H]5HT associated with particulate material, and also decreased the amount of [ 3 H]5HT recovered. 5. Serotonergic neurones possess specific mechanisms for the storage and axonal transport of 5HT which are absent in cholinergic cells.