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Effect of cations on the blood platelet release reaction
Author(s) -
Sneddon J. M.,
Williams K. I.
Publication year - 1973
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1973.sp010408
Subject(s) - platelet , chemistry , divalent , thrombin , trypsin , incubation , secretion , chelation , enzyme , biophysics , biochemistry , inorganic chemistry , biology , immunology , organic chemistry
1. Stimulus‐secretion phenomena in blood platelets incubated with trypsin or thrombin have been investigated. 2. Secretion occurs in at least two stages: ( a ) induction, obtained when the platelets are incubated with proteolytic enzymes in the presence of the chelating agent EDTA, and ( b ) release, which occurs with the subsequent addition of Ca 2+ to the incubation medium. 3. Ca 2+ was the only essential divalent cation required for release. Ba 2+ and Mg 2+ inhibited release and Sr 2+ could only partially substitute for Ca 2+ . 4. Blood platelets exhibited recovery from enzymic perturbation by trypsin or thrombin by a mechanism that was temperature dependent, but did not require the synthesis of new protein. 5. The results are discussed in relationship to stimulus—secretion in other cells.