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Active transport of 131 I across the blood—brain barrier
Author(s) -
Davson Hugh,
Hollingsworth Jillian R.
Publication year - 1973
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1973.sp010310
Subject(s) - blood–brain barrier , physics , chemistry , biophysics , neuroscience , central nervous system , biology
The ventricular space of rabbits was perfused with a low‐viscosity silicone oil for the purpose of (1) collecting freshly secreted cerebrospinal fluid (c.s.f.) uninfluenced by diffusional exchanges with the brain and (2) studying passage of solutes from the blood into the brain, uncomplicated by exchanges with c.s.f. The freshly secreted c.s.f. appeared as fine droplets suspended in the less dense silicone, and accumulated at the bottom of the collected silicone. Studies on the penetration of 24 Na from blood into this fluid indicated that considerable exchanges with the brain had occurred between its secretion and collection, in spite of this method of collection. The second objective was attained, in that the exchanges between the freshly secreted fluid and the brain were quantitatively insufficient to affect the measure of kinetics of uptake by brain from the blood. In consequence, it was possible to demonstrate unequivocally that the increased uptake by brain of 131 I, when treated with perchlorate, was due to inhibition of an active process occurring across the blood—brain barrier. Other studies, involving ventriculo‐cisternal perfusion with artificial c.s.f., lent further support to this concept. 131 I distribution is some 32% of the brain weight, a figure close to the ‘chloride‐space’.