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Blood volume determination in the mouse
Author(s) -
Riches A. C.,
Sharp J. G.,
Thomas D. Brynmor,
Smith S. Vaughan
Publication year - 1973
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1973.sp010086
Subject(s) - blood volume , plasma volume , volume (thermodynamics) , hematocrit , venous blood , red cell , red blood cell , albumin , body weight , chemistry , medicine , endocrinology , quantum mechanics , physics
1. The blood volume of the mouse has been measured using 59 Fe‐labelled red cells to determine the red cell volume and 131 I‐labelled human serum albumin to determine the plasma volume. 2. Values for the blood volume of 95·0 ± 1·5, 96·3 ± 2·7 and 84·7 ± 1·2 ml./kg body wt. were found for CSI female, CBA female and CBA male mice respectively. 3. A marked discrepancy was observed between the venous (cardiac) haematocrit and the whole body haematocrit. 4. The blood volume of the mouse must be determined from the red cell volume and the plasma volume, measured using appropriate labels, and not from the red cell volume or the plasma volume using the venous haematocrit.