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Absorption of palmitic, stearic and oleic acids in the sheep in the presence or absence of bile and/or pancreatic juice
Author(s) -
Harrison F. A.,
Leat W. M. F.
Publication year - 1972
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1972.sp009956
Subject(s) - stearic acid , oleic acid , chemistry , pancreatic juice , palmitic acid , absorption (acoustics) , food science , biochemistry , pancreas , organic chemistry , materials science , fatty acid , composite material
1. The absorption of 3 H‐ and 14 C‐labelled palmitic, stearic and oleic acids under control conditions and in the absence of bile and/or pancreatic juice has been studied in sheep prepared with intestinal cannulae and reentrant fistulation of the thoracic lymph duct. 2. Under control conditions the percentage recoveries in thoracic duct lymph of fatty acids introduced into the duodenum were: stearic acid 59·6 ± 3·2 (mean ± S.E. of mean), palmitic acid 71·1 ± 1·2 and oleic acid 80·4 ± 1·9. 3. At the peak of absorption over 90% of the radioactivity was located in the triglyceride fraction. 4. It was calculated that between 1900 and 3800 ml. lymph containing up to 20 g lipid flowed daily from the thoracic duct. The major fatty acids of lymph triglycerides were C 16:0 (26%), C 18:0 (39%) and C 18:1 (19%). 5. In the absence of pancreatic juice, but in the presence of bile, the absorption of stearic, palmitic and oleic acids was 0·8, 4·–8·5 and 26% respectively of the dose injected into the duodenum. In the absence of bile but presence of pancreatic juice corresponding values were 0·1–0·5, 1·8 and 7·7%. 6. In the absence of pancreatic juice the total lipid content of thoracic duct lymph fell from 800 to 80 mg% within 4 hr. When bile was also absent the lipid content of lymph fell further to 50 mg%. 7. It is concluded that pancreatic juice functions in the lumen of the intestine probably through the production from biliary lecithin of the lysolecithin necessary for the optimum absorption of lipids.