Premium
The influence of electrolytes on the volume of non‐metabolizing renal cortical cells
Author(s) -
McIver D. J. L.,
Raine A. E. G.
Publication year - 1972
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1972.sp009955
Subject(s) - electrolyte , swelling , chemistry , peg ratio , incubation , polyethylene glycol , renal cortex , volume (thermodynamics) , biophysics , chromatography , biochemistry , kidney , endocrinology , biology , chemical engineering , electrode , finance , quantum mechanics , engineering , economics , physics
1. The metabolism of rat renal cortex slices was inhibited by iodoacetate and anoxia, and swelling was prevented by the presence in the medium of 7·2 g polyethylene glycol 6000/100 ml. (referred to as PEG medium). 2. Slices were incubated for up to 12 hr in PEG medium, and in PEG media containing 440 m‐osmole/kg H 2 O of an electrolyte (LiCl, NaCl, KCl or RbCl), or a non‐electrolyte (glucose). 3. It was concluded that the slices in all media were at equilibrium with the medium after incubation for 8 hr. 4. Slices in the medium containing glucose reached the same equilibrium water content as those in the PEG medium, but slices in all the electrolyte media had significantly lower equilibrium water contents, although these did not differ significantly from each other. 5. It is suggested that the results demonstrate a non‐specific effect of electrolytes on the swelling of non‐metabolizing cells.