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The effect of carbohydrates on the intestinal potentials of Cryptochiton stelleri
Author(s) -
Lawrence A. L.,
Mailman D. S.,
Puddy R. E.
Publication year - 1972
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1972.sp009953
Subject(s) - phlorizin , galactose , fructose , medicine , small intestine , monosaccharide , sugar , potential difference , chemistry , endocrinology , biology , anatomy , glucose transporter , biochemistry , electrode , insulin
1. The effect of various monosaccharides on the potential difference across the intestine of the invertebrate, Cryptochiton stelleri , was studied using an everted sac technique. 2. D ‐Glucose, when present in the mucosal solution, increased the transmural potential across the anterior intestine but had no effect on electrical potentials across the posterior intestine. 3. D ‐Galactose, when present in the mucosal solution, increased the transmural potential across the posterior intestine but had no effect on electrical potentials across the anterior intestine. 4. D ‐Fructose and D ‐mannose, metabolizable but non‐transported sugars, increased anterior intestinal potentials but not posterior intestinal potentials. 5. 3‐ O ‐methylglucose, an actively transported (across the anterior intestine) but non‐metabolizable sugar, did not alter potentials across either the anterior or posterior intestine. 6. The addition of D ‐glucose to the serosal solution increased anterior intestinal potentials, but the serosal addition of D ‐galactose did not affect posterior intestinal potentials. 7. NaF, phlorizin and anoxia prevented the increase in potential across the anterior intestine and phlorizin (10 −4 but not 10 −6 M ) and NaF but not anoxia prevented the increase in potential across the posterior intestine. 8. It was suggested that the effect of glucose on anterior intestinal potentials was a metabolic effect, whereas the effect of galactose on posterior intestinal potentials was related to the active transport of that sugar from mucosa to serosa.