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Pancreatic acinar cells: measurement of membrane potential and miniature depolarization potentials
Author(s) -
Dean P. M.,
Matthews E. K.
Publication year - 1972
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1972.sp009926
Subject(s) - depolarization , membrane potential , physostigmine , biophysics , stimulation , chemistry , electrophysiology , acetylcholine , medicine , endocrinology , biology
1. Intracellular recordings of membrane potentials have been made in vitro from the exocrine acinar cells of the mouse pancreas using glass micro‐electrodes. 2. The mean membrane potential of the acinar cells was −41·2 mV. Spontaneous miniature depolarization potentials of 0·5–5·0 mV amplitude and occurring at random intervals were often observed superimposed upon the resting membrane potential. Atropine 2·8 × 10 −6 M decreased, and physostigmine 1·23 × 10 −5 M increased, the frequency and amplitude of the miniature potentials. 3. Electrical stimulation of the pancreatic nerves depolarized the acinar cells. Acetyl β‐methylcholine 10 −6 M produced a sustained depolarization. Atropine 1·4 × 10 −6 M blocked the depolarization to both electrical stimulation and acetyl β‐methylcholine. 4. Pancreozymin depolarized the exocrine acinar cells but secretin did not. 5. These results suggest that the miniature depolarization potentials are of cholinergic origin and that depolarization of the pancreatic exocrine cells by nerve stimulation or pancreozymin may trigger secretion.