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High potassium and low potassium erythrocytes in cattle
Author(s) -
Christinaz P.,
Schatzmann H. J.
Publication year - 1972
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1972.sp009901
Subject(s) - potassium , ouabain , breed , mole , microbiology and biotechnology , chemistry , membrane , biology , medicine , endocrinology , biochemistry , sodium , zoology , organic chemistry
1. The K and Na concentrations in red blood cells ( R.B.C. ) of 251 animals of the Simmenthal breed and thirty‐two animals of other cattle breeds were measured. [Na] cells +[K] cells was 89·4 m‐mole/l. cells. [K] cells varied between 7 and 70 and [Na] cells varied in the inverse sense between 15 and 87 m‐mole/l. cells. 2. The frequency distribution of animals according to K content of R.B.C.S , which could best be fitted by two superimposed Gaussian curves, suggests that there are two distinct types of cells (high K (HK) cells and low K (LK) cells). Animals with HK cells were considerably less frequent than animals with LK cells. 3. Differences in breed, age or sex do not account for the difference in cation content of R.B.C.S . 4. Cold stored or PCMBS‐treated HK cells show a more vigorous cation pump activity than equally treated LK cells. 5. At a Na concentration of 100 m M and a K concentration of 10 m M isolated haemoglobin‐free membranes prepared from HK cells exhibit a higher activity of Na+K stimulated (ouabain inhibitable) ATPase activity per mg of protein than membranes from LK cells.