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Radio‐immunoassay of gastrin in human plasma
Author(s) -
Ganguli P. C.,
Hunter W. M.
Publication year - 1972
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1972.sp009718
Subject(s) - gastrin , achlorhydria , medicine , endocrinology , pernicious anaemia , zollinger ellison syndrome , antibody , immunoassay , gastrointestinal hormone , gastric acid , volunteer , chemistry , hormone , gastroenterology , stomach , peptide hormone , secretion , biology , immunology , agronomy
1. A radio‐immunoassay for gastrin has been developed using partially purified porcine gastrin to raise antibodies and highly purified natural porcine gastrin I for radio‐iodination with 125 I. The separation of antibody‐bound from free hormone was performed by a double‐antibody method. 2. In this assay highly purified natural porcine gastrin I, synthetic human gastrin I, radio‐iodinated porcine gastrin I, gastrin in the plasma of a healthy volunteer, a patient with pernicious anaemia and another patient with the Zollinger—Ellison syndrome were immunologically identical. 3. The fasting plasma gastrin concentration of fourteen gastric ulcer patients was significantly higher than that of the 113 hospital controls with no history of gastro‐intestinal disease, while twenty‐seven duodenal ulcer patients had gastrin levels within the normal range. 4. Plasma gastrin concentration was significantly elevated in pernicious anaemia (fifty‐one patients), achlorhydria (thirty‐three patients), hypochlorhydria (eleven patients) and in nine patients with histologically proven Zollinger‐Ellison syndrome. 5. In human volunteers a protein meal stimulated endogenous gastrin release while a carbohydrate meal did not. Atropine sulphate I.M. , and hydrochloric acid orally, produced a significant fall in the level of circulating gastrin.