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Competitive interaction of β‐guanidino propionic acid and γ‐aminobutyric acid on the muscle fibre of the crayfish
Author(s) -
Feltz Anne
Publication year - 1971
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1971.sp009531
Subject(s) - aminobutyric acid , biophysics , conductance , crayfish , chemistry , membrane , gaba receptor , desensitization (medicine) , gamma aminobutyric acid , receptor , antagonist , gabaa receptor , medicine , stereochemistry , biochemistry , biology , ecology , mathematics , combinatorics
1. The interaction of β‐guanidino propionic acid and γ‐aminobutyric acid has been investigated on the crayfish muscle by measurement of the membrane conductance. 2. The increase in membrane conductance produced by GABA is depressed by β‐guanidino propionic acid. This antagonist acts in a competitive way as shown by the shift of log dose—response curves and by the results of tests for competitive interaction (method of Schild (1947); Lineweaver and Burk's plot). 3. Perfusion of GABA was shown to induce a receptor desensitization. As a consequence, the slope of the dose—response curve plotted in Hill's co‐ordinates increases when care is taken to minimize this phenomenon. The significance of this slope for establishing the real number of GABA molecules binding with the GABA receptor is discussed.