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Transfer of alanine isomers by rat small intestine
Author(s) -
de la Noüe J.,
Newey H.,
Smyth D. H.
Publication year - 1971
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1971.sp009421
Subject(s) - alanine , sarcosine , chemistry , methionine , biochemistry , stereochemistry , amino acid , glycine
1. A study was made of the transfer of L ‐alanine, D ‐alanine and β‐alanine by sacs of everted small intestine of the rat. 2. D ‐Alanine and β‐alanine are transported against a concentration gradient and in this respect resemble L ‐alanine. 3. Transfer of all three isomers was stimulated in presence of glucose, but stimulation was much greater with D ‐alanine and β‐alanine. 4. Transfer of all three isomers was inhibited by galactose. 5. The effect of hexoses on amino acid transfer was relatively independent of the effect on fluid transfer. 6. L ‐Methionine inhibited transfer of the three isomers in descending order, L ‐alanine, D ‐alanine and β‐alanine, while the magnitude of sarcosine inhibition was in the reverse order. 7. At the concentration used (1 m M ), L ‐alanine was transferred mostly by the methionine carrier, and β‐alanine mostly by the sarcosine carrier. D ‐Alanine occupied an intermediate position.