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The release of a renally active substance by perfused rat hearts
Author(s) -
Lockett Mary F.,
Retallack R. W.
Publication year - 1971
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1971.sp009353
Subject(s) - aldosterone , bicarbonate , chemistry , medicine , endocrinology , secretion , biology , biochemistry
1. A substance (HS) which resembles the 18‐monoacetate of D ‐aldosterone (18‐MA) in its biological actions is released by perfused rat hearts, HS is assayed in terms of activity equivalent to synthetic 18‐MA. 2. Isolated rat hearts perfused with modified Krebs bicarbonate Ringer without recirculation secrete progressively less and less HS. 3. Addition of D ‐aldosterone to recirculating perfusate supplying a heart no longer secreting measurable quantities of HS causes a rapid and very marked increase in the output of HS from the heart. If radioactively labelled D ‐aldosterone is supplied the HS synthesized is radioactively labelled. No measurable amounts of HS are formed from 18‐hydroxycorticosterone. 4. Chromatographic differentiation between 18‐MA and HS, initially difficult, has been achieved. 5. The rate of secretion of HS from the hearts of adrenalectomized animals is markedly reduced: synthesis of HS is immediately resumed when these hearts are supplied with D ‐aldosterone, but at a subnormal rate.