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The relation between L‐methionine uptake and sodium in rat small intestine in vitro
Author(s) -
Newey H.,
Rampone A. J.,
Smyth D. H.
Publication year - 1970
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1970.sp009292
Subject(s) - methionine , intracellular , mannitol , sodium , chemistry , in vitro , small intestine , jejunum , biochemistry , amino acid , organic chemistry
1. Uptakes of L ‐methionine and mannitol by rat jejunum in vitro were measured over test periods from 5 to 120 sec after 30 min pre‐test periods in the presence or absence of Na. 2. The initial stage in methionine uptake was dependent on the presence of Na + and to a lesser extent on the K + concentration. In contrast mannitol uptake was independent of Na and K. 3. The initial stage in methionine uptake can be reactivated 30–60% within 5 sec by replacing an Na‐deficient intestine into an Na‐containing medium. 4. Initial methionine uptake was greater with a normal intracellular and low medium Na concentration than with a high medium and low intracellular Na concentration. It is suggested that the intracellular Na concentration is a critical factor, more important than the Na gradient, in determining the rate of amino acid transfer across the luminal membrane.