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Entry of decamethonium in rat muscle studied by autoradiography
Author(s) -
Creese R.,
Maclagan Jennifer
Publication year - 1970
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1970.sp009215
Subject(s) - decamethonium , chemistry , anatomy , medicine , biochemistry , receptor
1. When a steady plasma level of decamethonium was maintained by infusion in rats, the labelled compound became concentrated in the region of the end‐plate of skeletal muscle, as shown by scintillation counting. 2. The distribution of tritium‐labelled decamethonium in single muscle fibres was studied by autoradiography of frozen sections, with resolution less than 1 μ. 3. After intravenous injection of a dose of decamethonium which produced partial paralysis it was shown that the labelled compound had entered muscle fibres in the region of the end‐plate, and for several hundred microns on either side of the end‐plate. 4. Entry of decamethonium could be demonstrated as early as 30 sec after intra‐arterial injection. There was no evidence of any redistribution of labelled drug for a period of 2 hr after the initial entry. 5. Previous administration of tubocurarine markedly reduced the entry of labelled decamethonium.