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Light scattering and birefringence changes during activity in the electric organ of Electrophorus electricus
Author(s) -
Cohen L. B.,
Hille B.,
Keynes R. D.
Publication year - 1969
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1969.sp008876
Subject(s) - electrophorus , birefringence , repolarization , scattering , light scattering , millisecond , stimulus (psychology) , materials science , optics , chemistry , biophysics , electrophysiology , physics , nuclear magnetic resonance , biology , medicine , biochemistry , psychology , torpedo , receptor , acetylcholine receptor , astronomy , psychotherapist
1. In an attempt to obtain information about structural changes related to electrical activity in Electrophorus electroplates, we have determined the size and time course of the changes in light scattering and in bire‐fringence that occur during and after the discharge of the electric organ. 2. The changes in light intensity detected with a photomultiplier were never greater than 0·2% for a single discharge, and were often much smaller than this, but records with an acceptable ratio of signal to noise could be obtained by signal‐averaging techniques. 3. A single stimulus led to a decrease, then an increase, and finally another decrease in the light scattered by slices of the main electric organ. These three phases were designated E1, E2 and E3. 4. E1 started at the beginning of the action potential, and its peak was reached at the same time as the completion of repolarization, even when the repolarization was delayed by cooling or hastened by drawing larger currents from the tissue. 5. E2 was proportional to the integral of the current flowing through the slice of electric organ, and may arise from the swelling and shrinking of the tubules that stud the faces of the electroplates. It developed within a millisecond or two of the start of an applied current, and lasted for about 100 msec. 6. E3 was a variable decrease in scattering that lasted for some seconds. 7. A stimulus also led to a transient increase in the birefringence of the electric organ. The optical change followed the change in electrical potential across the innervated faces of the electroplates with a delay of somewhat under 50 μsec. 8. This voltage‐dependent change in birefringence may arise from a Kerr effect (electric birefringence) in the membrane or from compression of the membrane.