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Some physiological properties of identified mammalian neuroglial cells
Author(s) -
Dennis M. J.,
Gerschenfeld H. M.
Publication year - 1969
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1969.sp008860
Subject(s) - iontophoresis , biophysics , neuroglia , membrane potential , pipette , optic nerve , chemistry , membrane , electrophysiology , fluorescence , anatomy , neuroscience , biology , central nervous system , biochemistry , physics , quantum mechanics
Mammalian glial cells were identified and studied in the optic nerves of anaesthetized rats. Cells with membrane potentials of 77–85 mV were located in the optic nerve with capillary micropipettes. These were shown to be neuroglia by iontophoretic injection of a fluorescent dye through the recording electrode, followed by histological verification of the location of the dye. No distinction was made between astroglia and oligodendroglia. Neuroglial cells gave no impulse activity. Their membrane potential was studied in isolated optic nerves by varying the ionic composition of the bathing fluid. The glial membrane potential depends predominantly on a transmembrane gradient of potassium ions.