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Action of amphotericin B on the toad bladder: evidence for sodium transport along two pathways
Author(s) -
Bentley P. J.
Publication year - 1968
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1968.sp008531
Subject(s) - toad , sodium , chemistry , aldosterone , endocrinology , medicine , organic chemistry
1. The relationship of short‐circuit current (SCC, sodium transport) and potential difference (p.d.) across the toad bladder to the concentration of sodium on the mucosal side was measured. Maximal values were attained when the Na concentration was 10 m M for SCC and 30 m M for p.d. 2. Amphotericin B increased SCC across bladders bathed on their mucosal surface with isotonic (115 m M ) NaCl solution and this effect was not inhibited by cyanide and iodoacetate. The osmotic permeability of the bladder was also increased by amphotericin B. 3. Vasopressin and aldosterone increased sodium transport from solutions of low Na concentration, but amphotericin B was ineffective until a level of about 40‐60 m M was present. 4. The evidence suggests two pathways for sodium transport across the bladder distinguished by differences in their electrochemical gradients with the fluid at the mucosal side.