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The release of 5‐hydroxytryptamine from rabbit small intestine in vitro
Author(s) -
Gwee M. C. E.,
Yeoh T. S.
Publication year - 1968
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1968.sp008437
Subject(s) - nialamide , perfusion , small intestine , peristalsis , chemistry , reserpine , lumen (anatomy) , medicine , in vitro , serotonin , rabbit (cipher) , anatomy , endocrinology , biology , biochemistry , statistics , receptor , mathematics
1. A method is described by which the lumen of intestinal segments may be perfused separately and simultaneously under identical conditions of perfusion pressure and temperature, and the rate of appearance of 5‐hydroxytryptamine (5‐HT) into the serosal fluid measured. 2. 5‐HT appeared in the serosal fluid from quiescent segments of rabbit intestine at a rate of about 4·0 ng/cm intestine/hr and about 14·0 ng/cm intestine/hr from segments performing active peristaltic movements. 3. For both quiescent and active segments, when the perfusion fluid contained a monoamine oxidase inhibitor, nialamide (Niamid), the rate of appearance of 5‐HT was increased and if, in addition, 5‐HT was present in the perfusion fluid the rate of appearance of 5‐HT in the serosal fluid was even greater. 4. The rate of appearance of 5‐HT from the intestine of rabbits pretreated with reserpine was about 0·8 ng/cm intestine/hr and Niamid only slightly increased this rate. However, if 5‐HT as well as Niamid were present the rate of appearance of 5‐HT was about 6·0 ng/cm intestine/hr. 5. Possible explanations for these findings are discussed.