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Arterial carbon dioxide and bicarbonate rather than pH regulate cerebral blood flow in the setting of acute experimental metabolic alkalosis
Author(s) -
Caldwell Hannah G.,
Howe Connor A.,
Chalifoux Carter J.,
Hoiland Ryan L.,
Carr Jay M. J. R.,
Brown Courtney V.,
Patrician Alexander,
Tremblay Joshua C.,
Panerai Ronney B.,
Robinson Thompson G.,
Minhas Jatinder S.,
Ainslie Philip N.
Publication year - 2021
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jp280682
Subject(s) - hypocapnia , hypercapnia , cerebral blood flow , bicarbonate , alkalosis , anesthesia , sodium bicarbonate , acidosis , arterial blood , medicine , chemistry
Key points We investigated the influence of arterial P C O 2( P aC O 2) with and without acutely elevated arterial pH and bicarbonate ([HCO 3 – ]) on cerebral blood flow (CBF) regulation in the internal carotid artery and vertebral artery. We assessed stepwise iso‐oxic alterations in P aC O 2(i.e. cerebrovascular CO 2 reactivity) prior to and following i.v. sodium bicarbonate infusion (NaHCO 3 – ) to acutely elevate arterial pH and [HCO 3 – ]. Total CBF was unchanged irrespective of a higher arterial pH at each matched stage of P aC O 2, indicating that CBF is acutely regulated by P aC O 2rather than arterial pH. The cerebrovascular responses to changes in arterial H + /pH were altered in keeping with the altered relationship between P aC O 2and H + /pH following NaHCO 3 – infusion (i.e. changes in buffering capacity). Total CBF was ∼7% higher following NaHCO 3 – infusion during isocapnic breathing providing initial evidence for a direct vasodilatory influence of HCO 3 – independent of P aC O 2levels.Abstract Cerebral blood flow (CBF) regulation is dependent on the integrative relationship between arterial P C O 2( P aC O 2), pH and cerebrovascular tone; however, pre‐clinical studies indicate that intrinsic sensitivity to pH, independent of changes in P aC O 2or intravascular bicarbonate ([HCO 3 – ]), principally influences cerebrovascular tone. Eleven healthy males completed a standardized cerebrovascular CO 2 reactivity (CVR) test utilizing radial artery catheterization and Duplex ultrasound (CBF); consisting of matched stepwise iso‐oxic alterations in P aC O 2(hypocapnia: –5, –10 mmHg; hypercapnia: +5, +10 mmHg) prior to and following i.v. sodium bicarbonate (NaHCO 3 – ; 8.4%, 50 mEq 50 mL –1 ) to elevate pH (7.408 ± 0.020 vs . 7.461 ± 0.030; P < 0.001) and [HCO 3 – ] (26.1 ± 1.4 vs . 29.3 ± 0.9 mEq L –1 ; P < 0.001). Absolute CBF was not different at each stage of CO 2 reactivity ( P = 0.629) following NaHCO 3 – , irrespective of a higher pH ( P < 0.001) at each matched stage of P aC O 2( P = 0.927). Neither hypocapnic (3.44 ± 0.92 vs . 3.44 ± 1.05% per mmHg P aC O 2; P = 0.499), nor hypercapnic (7.45 ± 1.85 vs . 6.37 ± 2.23% per mmHg P aC O 2; P = 0.151) reactivity to P aC O 2were altered pre‐ to post‐NaHCO 3 – . When indexed against arterial [H + ], the relative hypocapnic CVR was higher ( P = 0.019) and hypercapnic CVR was lower ( P = 0.025) following NaHCO 3 – , respectively. These changes in reactivity to [H + ] were, however, explained by alterations in buffering between P aC O 2and arterial H + /pH consequent to NaHCO 3 – . Lastly, CBF was higher (688 ± 105 vs . 732 ± 89 mL min –1 , 7% ± 12%; P = 0.047) following NaHCO 3 – during isocapnic breathing providing support for a direct influence of HCO 3 – on cerebrovascular tone independent of P aC O 2. These data indicate that in the setting of acute metabolic alkalosis, CBF is regulated by P aC O 2rather than arterial pH.