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New insights into ion channel‐dependent signalling during left‐right patterning
Author(s) -
Tajhya Rajeev,
Delling Markus
Publication year - 2019
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jp277835
Subject(s) - cilium , microbiology and biotechnology , motile cilium , notochord , ion channel , biology , anatomy , hedgehog signaling pathway , embryonic stem cell , neuroscience , gene , signal transduction , receptor , genetics , embryogenesis , embryo
The left‐right organizer (LRO) in the mouse consists of pit cells within the depression, located at the end of the developing notochord, also known as the embryonic node and crown cells lining the outer periphery of the node. Cilia on pit cells are posteriorly tilted, rotate clockwise and generate leftward fluid flow. Primary cilia on crown cells are required to interpret the directionality of fluid movement and initiate flow‐dependent gene transcription. Crown cells express PC1‐L1 and PC2, which may form a heteromeric polycystin channel complex on primary cilia. It is still only poorly understood how fluid flow activates the ciliary polycystin complex. Besides polycystin channels voltage gated channels like HCN4 and KCNQ1 have been implicated in establishing asymmetry. How this electrical network of ion channels initiates left‐sided signalling cascades and differential gene expression is currently only poorly defined.