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GABA is a modulator, rather than a classical transmitter, in the medial nucleus of the trapezoid body–lateral superior olive sound localization circuit
Author(s) -
Fischer Alexander U.,
Müller Nicolas I. C.,
Deller Thomas,
Del Turco Domenico,
Fisch Jonas O.,
Griesemer Désirée,
Kattler Kathrin,
Maraslioglu Ayse,
Roemer Vera,
XuFriedman Matthew A.,
Walter Jörn,
Friauf Eckhard
Publication year - 2019
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jp277566
Subject(s) - trapezoid body , gabaergic , inhibitory postsynaptic potential , neuroscience , neurotransmission , excitatory postsynaptic potential , glycine receptor , superior olivary complex , gamma aminobutyric acid , biology , synapse , patch clamp , nucleus , chemistry , receptor , cochlear nucleus , electrophysiology , glycine , biochemistry , amino acid
Key points The lateral superior olive (LSO), a brainstem hub involved in sound localization, integrates excitatory and inhibitory inputs from the ipsilateral and the contralateral ear, respectively. In gerbils and rats, inhibition to the LSO reportedly shifts from GABAergic to glycinergic within the first three postnatal weeks. Surprisingly, we found no evidence for synaptic GABA signalling during this time window in mouse LSO principal neurons. However, we found that presynaptic GABA B Rs modulate Ca 2+ influx into medial nucleus of the trapezoid body axon terminals, resulting in reduced synaptic strength. Moreover, GABA elicited strong responses in LSO neurons that were mediated by extrasynaptic GABA A Rs. RNA sequencing revealed highly abundant δ subunits, which are characteristic of extrasynaptic receptors. Whereas GABA increased the excitability of neonatal LSO neurons, it reduced the excitability around hearing onset. Collectively, GABA appears to control the excitability of mouse LSO neurons via extrasynaptic and presynaptic signalling. Thus, GABA acts as a modulator, rather than as a classical transmitter.Abstract GABA and glycine mediate fast inhibitory neurotransmission and are coreleased at several synapse types. Here we assessed the contribution of GABA and glycine in synaptic transmission between the medial nucleus of the trapezoid body (MNTB) and the lateral superior olive (LSO), two nuclei involved in sound localization. Whole‐cell patch‐clamp experiments in acute mouse brainstem slices at postnatal days (P) 4 and 11 during pharmacological blockade of GABA A receptors (GABA A Rs) and/or glycine receptors demonstrated no GABAergic synaptic component on LSO principal neurons. A GABAergic component was absent in evoked inhibitory postsynaptic currents and miniature events. Coimmunofluorescence experiments revealed no codistribution of the presynaptic GABAergic marker GAD65/67 with gephyrin, a postsynaptic marker for GABA A Rs, corroborating the conclusion that GABA does not act synaptically in the mouse LSO. Imaging experiments revealed reduced Ca 2+ influx into MNTB axon terminals following activation of presynaptic GABA B Rs. GABA B R activation reduced the synaptic strength at P4 and P11. GABA appears to act on extrasynaptic GABA A Rs as demonstrated by application of 4,5,6,7‐tetrahydroisoxazolo[5,4‐c]pyridin‐3‐ol, a δ‐subunit‐specific GABA A R agonist. RNA sequencing showed high mRNA levels for the δ‐subunit in the LSO. Moreover, GABA transporters GAT‐1 and GAT‐3 appear to control extracellular GABA. Finally, we show an age‐dependent effect of GABA on the excitability of LSO neurons. Whereas tonic GABA increased the excitability at P4, leading to spike facilitation, it decreased the excitability at P11 via shunting inhibition through extrasynaptic GABA A Rs. Taken together, we demonstrate a modulatory role of GABA in the murine LSO, rather than a function as a classical synaptic transmitter.