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Activation of voltage‐dependent K + channels strongly limits hypoxia‐induced elevation of [Ca 2+ ] i in rat carotid body glomus cells
Author(s) -
Wang Jiaju,
Kim Donghee
Publication year - 2017
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jp275275
Subject(s) - glomus cell , carotid body , bk channel , membrane potential , hypoxia (environmental) , biophysics , chemistry , endocrinology , medicine , biology , stimulation , oxygen , organic chemistry
Key points We studied the role of the large‐conductance Ca 2+ ‐activated K + channel (BK) and voltage‐dependent K + channels (Kv) on [Ca 2+ ] i responses to a wide range of hypoxia at different resting cell membrane potential ( E m ). BK/Kv were mostly closed at rest in normoxia. BK/Kv became basally active when cells were depolarized by elevated [KCl] o (>12 m m ). Regardless of whether BK/Kv were closed or basally open, hypoxia‐induced elevation of [Ca 2+ ] i was enhanced 2‐ to 3‐fold by inhibitors of BK/Kv. Hypoxia‐induced elevation of [Ca 2+ ] i was enhanced ∼2‐fold by an inhibitor of Kv2, a major Kv in rat glomus cells. Hypoxia did not inhibit BK in inside‐out patches. Our study supports a scheme in which activation of BK/Kv strongly limits the magnitude of hypoxia‐induced [Ca 2+ ] i rise, with Kv having a much greater effect than BK.Abstract Large‐conductance K Ca (BK) and other voltage‐dependent K + channels (Kv) are highly expressed in carotid body (CB) glomus cells, but their role in hypoxia‐induced excitation is still not well defined and remains controversial. We addressed this issue by studying the effects of inhibitors of BK (IBTX) and BK/Kv (TEA/4‐AP) on [Ca 2+ ] i responses to a wide range of hypoxia at different levels of resting cell membrane potential ( E m ). IBTX and TEA/4‐AP did not affect the basal [Ca 2+ ] i in isolated glomus cells bathed in 5 m m KCl o , but elicited transient increases in [Ca 2+ ] i in cells that were moderately depolarized (11–20 mV) by elevation of [KCl] o (12–20 m m ). Thus, BK and Kv were mostly closed at rest and activated by depolarization. Four different levels of hypoxia (mild, moderate, severe, anoxia) were used to produce a wide range of [Ca 2+ ] i elevation (0–700 n m ). IBTX did not affect the rise in [Ca 2+ ] i , but TEA/4‐AP strongly (∼3‐fold) enhanced [Ca 2+ ] i rise by moderate and severe levels of hypoxia. Guangxitoxin, a Kv2 blocker, inhibited the whole‐cell current by ∼50%, and enhanced 2‐fold the [Ca 2+ ] i rise elicited by moderate and severe levels of hypoxia. Anoxia did not directly affect BK, but activated BK via depolarization. Our findings do not support the view that hypoxia inhibits BK/Kv to initiate or maintain the hypoxic response. Rather, our results show that BK/Kv are activated as glomus cells depolarize in response to hypoxia, which then limits the rise in [Ca 2+ ] i . Inhibition of Kv may provide a mechanism to enhance the chemosensory activity of the CB and ventilation.