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Ciliary beat frequency controlled by oestradiol and progesterone during ovarian cycle in guinea‐pig Fallopian tube
Author(s) -
Nishimura Ayumi,
Sakuma Ko,
Shimamoto Chikao,
Ito Shigenori,
Nakano Takashi,
Daikoku Eriko,
Ohmichi Masahide,
Ushiroyama Takahisa,
Ueki Minoru,
Kuwabara Hiroko,
Mori Hiroshi,
Nakahari Takashi
Publication year - 2010
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1113/expphysiol.2010.052555
Subject(s) - endocrinology , medicine , luteal phase , chemistry , guinea pig , follicular phase , biology
The ciliary beat frequency (CBF) of guinea‐pig fimbria during the ovarian cycle was measured by video microscopy using a high‐speed camera (500 Hz). In the follicular phase, with increasing concentrations of β‐oestradiol ([βE 2 ]) and a low concentration of progesterone ([PRG]), CBF increased from 13.5 to 16 Hz. In the ovulatory phase, with further increase of [βE 2 ], CBF decreased gradually from 16 to 13.5 Hz. In the early luteal phase, with low [PRG] and [βE 2 ], CBF increased to ∼17 Hz; however, in the middle luteal phase, with increasing [PRG], CBF decreased (∼12 Hz), and in the late luteal phase, with decreasing [PRG], CBF increased to ∼15 Hz. Then, in the resting phase, with low [βE 2 ] and [PRG], CBF decreased immediately to ∼14 Hz. The CBF of the fimbria was measured in guinea‐pigs treated with β‐oestradiol benzoate (βE 2 B) or medroxyprogesterone (mPRG). A low dose of βE 2 B increased CBF to ∼14.5 Hz, whereas a high dose decreased it to ∼11 Hz. A βE 2 receptor blocker, ICI‐182,780, abolished the βE 2 B‐induced CBF changes and maintained CBF at ∼12.0 Hz. Medroxyprogesterone decreased CBF to ∼12.5 Hz, and mifepristone (a PRG receptor blocker) abolished the mPRG‐induced CBF decrease and maintained CBF at ∼15 Hz. The addition of both blockers increased CBF to ∼18 Hz, suggesting that activation of βE 2 or PRG receptors decreases the CBF of the fimbria. In conclusion, a moderate [βE 2 ] increase maintains a high CBF (15.5 Hz) in the follicular phase, and then further [βE 2 ] increase decreases CBF to 13.5 Hz in the ovulatory phase. In the early and late luteal phase, low [βE 2 ] and [PRG] increase CBF to 17 and 15 Hz, respectively, and in the middle luteal phase a high [PRG] decreases CBF (to ∼12 Hz). Thus, the CBF of the fimbria was controlled by signals via βE 2 and PRG receptors in guinea‐pigs.