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Angiotensin II stimulates water and NaCl intake through separate cell signalling pathways in rats
Author(s) -
Daniels Derek,
Mietlicki Elizabeth G.,
Nowak Erica L.,
Fluharty Steven J.
Publication year - 2009
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1113/expphysiol.2008.044446
Subject(s) - angiotensin ii , protein kinase c , chelerythrine , endocrinology , protein kinase a , kinase , medicine , chemistry , intracellular , stimulation , signal transduction , receptor , renin–angiotensin system , biology , pharmacology , microbiology and biotechnology , biochemistry , blood pressure
Angiotensin II (AngII) stimulation of water and NaCl intake is a classic model of the behavioural effects of hormones. In vitro studies indicate that the AngII type 1 (AT 1 ) receptor stimulates intracellular pathways that include protein kinase C (PKC) and mitogen‐activated protein (MAP) kinase activation. Previous studies support the hypotheses that PKC is involved in AngII‐induced water, but not NaCl intake and that MAP kinase plays a role in NaCl consumption, but not water intake, after injection of AngII. The present experiments test these hypotheses in rats using central injections of AngII in the presence or absence of a PKC inhibitor or a MAP kinase inhibitor. Pretreatment with the PKC inhibitor chelerythrine attenuated AngII‐induced water intake, but NaCl intake was unaffected. In contrast, pretreatment with U0126, a MAP kinase inhibitor, had no effect on AngII‐induced water intake, but attenuated NaCl intake. These data support the working hypotheses and significantly extend our earlier findings and those of others. Perhaps more importantly, these experiments demonstrate the remarkable diversity of peptide receptor systems and add support for the surprising finding that intracellular signalling pathways can have divergent behavioural relevance.