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Fibre type distribution and gene expression levels of both succinate dehydrogenase and peroxisome proliferator‐activated receptor‐γ coactivator‐1α of fibres in the soleus muscle of Zucker diabetic fatty rats
Author(s) -
Adachi Tetsuya,
Kikuchi Naoko,
Yasuda Koichiro,
Anahara Reiko,
Gu Ning,
Matsunaga Tetsuro,
Yamamura Tsubasa,
Mori Chisato,
Tsujimoto Gozoh,
Tsuda Kinsuke,
Ishihara Akihiko
Publication year - 2007
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1113/expphysiol.2006.035451
Subject(s) - endocrinology , medicine , laser capture microdissection , chemistry , type 2 diabetes , peroxisome proliferator activated receptor , receptor , coactivator , succinate dehydrogenase , soleus muscle , dehydrogenase , gene expression , mitochondrion , diabetes mellitus , biology , enzyme , skeletal muscle , biochemistry , gene , transcription factor
We have reported that a change in muscle fibre type distribution is present in two strains of diabetic rats (Otsuka Long–Evans Tokushima Fatty and Goto‐Kakizaki rats). In this study, we determined whether the change in soleus muscle fibre type distribution was caused by diabetes, using obese, diabetic (Zucker diabetic fatty, ZDF), obese, non‐diabetic (Zucker fatty, ZF) and non‐diabetic, non‐obese rats (Zucker lean, ZL). Moreover, we investigated whether the gene expression levels of metabolic key molecules, namely the transcriptional factors of metabolic genes, exemplified by peroxisome proliferator‐activated receptor‐γ coactivator‐1α (PGC‐1α), and the oxidative enzymes in mitochondria, exemplified by succinate dehydrogenase (SDH), were changed in type I and II muscle fibres in each type of rat, using the new technique of laser capture microdissection (LCM). Both plasma glucose and glucosylated haemoglobin levels were significantly higher in ZDF than in ZL and ZF rats. A lower percentage of type IIA fibres was observed in the muscles of ZDF rats than in those of ZL and ZF rats. The mRNA expression levels of SDH in type II fibres and of PGC‐1α in type I fibres were significantly lower in ZDF than in ZL and ZF rats as assessed by LCM and real‐time PCR analysis. We have shown, for the first time, that a lower percentage of type IIA fibres was observed in ZDF rats. We have also discovered that the expression levels of the oxidative metabolism‐related genes, PGC‐1α and SDH, decreased in type I and type II fibres, respectively, of ZDF rats.