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Nitric oxide and thyroid gland: modulation of cardiovascular function in autonomic‐blocked anaesthetized rats
Author(s) -
Fellet Andrea Lorena,
Arza Patricia,
Arreche Noelia,
Arranz Cristina,
Balaszczuk Ana Mar'a
Publication year - 2004
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1113/expphysiol.2004.027201
Subject(s) - endocrinology , medicine , chronotropic , thyroidectomy , heart rate , adrenalectomy , blood pressure , phenylephrine , nitric oxide , nitric oxide synthase , mean arterial pressure , chemistry , thyroid
We have previously reported that acute administration of N G ‐nitro‐ l ‐arginine methyl ester ( l ‐NAME) increases the mean arterial pressure (MAP) and heart rate (HR) in autonomic‐blocked (CAB) anaesthetized rats. In the present study we examined whether thyroid and adrenal glands are involved in these pressor and chronotropic responses. Sprague‐Dawley rats were studied after bilateral vagotomy and ganglionic blockade with hexamethonium (10 mg kg −1 ), and stabilization of MAP with infusion of phenylephrine (PE) (6 μg kg −1 min −1 ). The rats were divided into groups: L, CAB; PE, CAB + PE bolus (6 μg kg −1 ); L‐TX, thyroidectomy + CAB; L‐AX, adrenalectomy + CAB; TX, only thyroidectomy; C, CAB. L, L‐AX and L‐TX groups received a bolus of l ‐NAME (7.5 mg kg −1 ). Triiodothyronine (T3), thyroxin (T4) and thyrotropin (TSH) levels were measured in L and L‐TX rats before and after l ‐NAME administration. Reduced nicotamide adenine dinucleotide (NADPH) diaphorase activity was determined in heart and aorta of the TX group. The pressor response induced by l ‐NAME was similar in all groups. l ‐NAME‐induced‐tachycardia was associated with this rise in MAP. Adrenalectomy did not modify this chronotropic response, but it was attenuated by thyroidectomy. Thyroidectomy by itself decreased the circulating levels of T3 but it had no effect on the plasma levels of T4 and TSH. L and L‐TX groups showed similar levels of circulating T4 and TSH, meanwhile the plasma level of T3 decreased in the L group. Nitric oxide synthase (NOS) activity in atria as well as in aorta was greater in the TX group compared with C. When autonomic influences are removed, the thyroid gland modulates intrinsic heart rate via a mechanism that involves, at least in part, the nitric oxide pathway.