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The effect of hypoxia on pulmonary O 2 uptake, leg blood flow and muscle deoxygenation during single‐leg knee‐extension exercise
Author(s) -
DeLorey Darren S.,
Shaw Colin N.,
Shoemaker J. Kevin,
Kowalchuk John M.,
Paterson Donald H.
Publication year - 2004
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1113/expphysiol.2003.026864
Subject(s) - deoxygenation , hypoxia (environmental) , blood flow , cardiology , medicine , chemistry , anatomy , oxygen , biochemistry , organic chemistry , catalysis
The effect of hypoxic breathing on pulmonary O 2 uptake (VO 2p ), leg blood flow (LBF) and O 2 delivery and deoxygenation of the vastus lateralis muscle was examined during constant‐load single‐leg knee‐extension exercise. Seven subjects (24 ± 4 years; mean ± s.d. ) performed two transitions from unloaded to moderate‐intensity exercise (21 W) under normoxic and hypoxic (P ET O 2 = 60 mmHg) conditions. Breath‐by‐breath VO 2p and beat‐by‐beat femoral artery mean blood velocity (MBV) were measured by mass spectrometer and volume turbine and Doppler ultrasound (VingMed, CFM 750), respectively. Deoxy‐(HHb), oxy‐, and total haemoglobin/myoglobin were measured continuously by near‐infrared spectroscopy (NIRS; Hamamatsu NIRO‐300). VO 2p data were filtered and averaged to 5 s bins at 20, 40, 60, 120, 180 and 300 s. MBV data were filtered and averaged to 2 s bins (1 contraction cycle). LBF was calculated for each contraction cycle and averaged to 5 s bins at 20, 40, 60, 120, 180 and 300 s. VO 2p was significantly lower in hypoxia throughout the period of 20, 40, 60 and 120 s of the exercise on‐transient. LBF (l min −1 ) was approximately 35% higher ( P > 0.05) in hypoxia during the on‐transient and steady‐state of KE exercise, resulting in a similar leg O 2 delivery in hypoxia and normoxia. Local muscle deoxygenation (HHb) was similar in hypoxia and normoxia. These results suggest that factors other than O 2 delivery, possibly the diffusion of O 2, were responsible for the lower O 2 uptake during the exercise on‐transient in hypoxia.