Premium
Effect of calcitonin, elevated calcium and extracellular matrices on superoxide anion production by rat osteoclasts
Author(s) -
Datta HK,
Manning P,
Rathod H,
McNeil CJ
Publication year - 1995
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1113/expphysiol.1995.sp003880
Subject(s) - superoxide , extracellular , chemistry , calcium , calcitonin , osteoclast , biochemistry , biophysics , endocrinology , medicine , biology , in vitro , enzyme , organic chemistry
The electrochemical determination of superoxide anion generation by isolated mammalian osteoclasts was carried out by employing cytochrome c, immobilized at a surface‐modified gold electrode. Osteoclasts cultured on bone generated 15‐fold more superoxide anions than cells plated on plastic. Superoxide anion production by osteoclasts cultured on bone was almost completely abolished by salmon calcitonin, and partially abolished following exposure to elevated extracellular calcium. We therefore conclude that osteoclast activity is influenced substantially by the extracellular matrix.