Premium
Sodium and chloride transport across the rumen epithelium of cattle in vitro: effect of short‐chain fatty acids and amiloride
Author(s) -
Diernaes L,
Sehested J,
Moller PD,
Skadhauge E
Publication year - 1994
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1113/expphysiol.1994.sp003805
Subject(s) - amiloride , ussing chamber , rumen , chemistry , sodium , voltage clamp , chloride , clamp , biophysics , absorption (acoustics) , patch clamp , in vitro , biochemistry , fatty acid , analytical chemistry (journal) , chromatography , biology , membrane potential , materials science , organic chemistry , mechanical engineering , clamping , receptor , fermentation , engineering , composite material
Isolated mucosal sheets of cattle rumen were studied in vitro in Ussing chambers using a computer‐controlled voltage clamp. Unidirectional fluxes of Na+ and Cl‐ were measured in standard (short‐chain fatty acid (SCFA)‐free) or SCFA‐containing Ringer solution under zero voltage clamp conditions. Net NaCl absorption was observed under zero voltage clamp conditions, was stimulated by SCFAs and was not reduced by 1 mmol l‐1 amiloride, although short‐circuit current was significantly reduced by this concentration of the blocker. A constant coupling ratio of Na+ and Cl‐ absorption was observed.