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Expression of c‐fos in the ovine brain following different types of stress, or central administration of corticotrophin‐releasing hormone
Author(s) -
Vellucci SV,
Parrott RF
Publication year - 1994
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1113/expphysiol.1994.sp003757
Subject(s) - area postrema , endocrinology , medicine , supraoptic nucleus , hypothalamus , hormone , brainstem , nucleus , saline , c fos , insulin , corticotropin releasing hormone , chemistry , biology , central nervous system , gene expression , neuroscience , biochemistry , gene
Expression of c‐fos protein was examined in the brains of sheep (n = 2 per treatment) subjected to physical stress in a transport simulator, the psychological stress of isolation, insulin‐induced hypoglycaemia or intracerebroventricular (I.C.V.) injection of ovine corticotrophin‐releasing hormone (CRH). As control procedures, two sheep were given I.C.V. injections of saline and one animal received no treatment. Fos expression was induced in the hypothalamic paraventricular nucleus by transport simulation, isolation and hypoglycaemia, and also in the hypothalamic supraoptic nucleus by hypoglycaemia. Labelled nuclei were also seen in the brainstem in the region of the area postrema following transport simulation, isolation and insulin treatments. These results indicate that c‐fos expression in the ovine CNS is a useful marker for the identification of brain regions activated by stressors.