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Confocal analysis of fluorescent bead uptake by mouse Peyer's patch follicle‐associated M cells
Author(s) -
Porta C,
James PS,
Phillips AD,
Savidge TC,
Smith MW,
Cremaschi D
Publication year - 1992
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1113/expphysiol.1992.sp003662
Subject(s) - follicle , peyer's patch , chemistry , bovine serum albumin , fluorescence , confocal , antibody , microbiology and biotechnology , albumin , biology , antigen , biochemistry , immunology , endocrinology , physics , geometry , mathematics , quantum mechanics
Latex beads coated with secretory immunoglobulin (IgA) instilled into mouse intestinal loops are taken up by M cells present in Peyer's patch follicle‐associated epithelial tissue. Bead adsorption and uptake is greater at the edge compared with the apex of follicle domes. Coating beads with bovine serum albumin (BSA) causes a fourfold reduction in adsorption and a twentyfold reduction in uptake. Results demonstrate selectivity between adsorption and uptake and between the ability of different proteins to facilitate uptake.