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Voltage‐sensitive elevation of cytosolic [Ca2+] in guinea‐pig cardiac myocytes elicited by calcitonin gene‐related peptide
Author(s) -
Gill JS,
Moonga BS,
Huang CL,
Lu F,
Zaidi M,
Camm AJ
Publication year - 1992
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1113/expphysiol.1992.sp003661
Subject(s) - depolarization , calcitonin gene related peptide , cytosol , calcitonin , guinea pig , myocyte , medicine , endocrinology , peptide , chemistry , calcium , biophysics , biology , neuropeptide , biochemistry , receptor , enzyme
We have studied the effect of the 37‐amino acid cardioactive peptide, calcitonin gene‐related peptide (CGRP) on cytosolic [Ca2+] in guinea‐pig ventricular myocytes following depolarization. Cytosolic [Ca2+] was measured in single myocytes using fura‐2. The application of 20 mM K+ led to a transient rise of cytosolic [Ca2+] followed by an exponential decline. The subsequent application of 2 nM CGRP resulted in a marked increase in cytosolic [Ca2+]. In contrast, no such response was obtained without prior depolarization. The results suggest a basis for the cardiotropic effects of CGRP through an influence on cytosolic [Ca2+].