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Electrical resistances of cultured bovine arterial endothelium in solutions of various resistivities
Author(s) -
Turner MR
Publication year - 1992
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1113/expphysiol.1992.sp003640
Subject(s) - paracellular transport , endothelium , electrical resistivity and conductivity , anatomy , chemistry , medicine , biophysics , biology , endocrinology , membrane , physics , biochemistry , permeability (electromagnetism) , quantum mechanics
Relationships between the electrical resistance (Rm) of cultured endothelium and the resistivity (rho) of the bathing solution have been investigated, using eight monolayers of bovine arterial endothelium. Bathing solutions of differing rho were prepared by mixing culture medium and isosmolar (290 mM) sucrose in various proportions. Relationships between 1/Rm and 1/rho were linear, as predicted, with a mean 1/Rm intercept of zero. The slopes of the relationships give a mean fractional area per unit path length (Ap/delta chi) of 12.7 cm‐1 for the paracellular pathways of the cultured endothelium. This resembles previous estimates of Ap/delta chi for mammalian endothelium in arteries. The 1/Rm intercept of zero indicates that the major electrical conducting pathways of cultured arterial endothelium are paracellular.