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Involvement of carbonic anhydrase in ammonia flux across rumen mucosa in vitro
Author(s) -
Bodeker D,
Oppelland G,
Holler H
Publication year - 1992
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1113/expphysiol.1992.sp003614
Subject(s) - carbonic anhydrase , rumen , in vitro , ammonia , acetazolamide , chemistry , biochemistry , flux (metallurgy) , absorption (acoustics) , enzyme , biology , physiology , fermentation , organic chemistry , materials science , composite material
Carbonic anhydrase activity in mucosa of the sheep rumen wall was completely inhibited by acetazolamide and ethoxyzolamide. This inhibition significantly reduced ammonia flux across mucosal discs in vitro but was ineffective if short‐chain fatty acids were present in the bathing solution. The findings are discussed in relation to the mechanism of ruminal ammonia absorption.