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Osteoclasts from medullary bone of egg‐laying Japanese quail do not express the putative calcium ‘receptor’
Author(s) -
Bascal ZA,
Moonga BS,
Dacke CG,
Zaidi M
Publication year - 1992
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1113/expphysiol.1992.sp003610
Subject(s) - quail , ionomycin , calcium , medicine , medullary cavity , endocrinology , extracellular , biology , receptor , chemistry , anatomy , microbiology and biotechnology
The present study reports the contrasting effects of extracellular calcium ([Ca2+]e) elevation on cytosolic free calcium levels ([Ca2+]i) of osteoclasts, freshly isolated either from medullary bone of the egg‐laying Japanese quail or from rat cortical bone. [Ca2+]i was measured in single osteoclasts using the Ca(2+)‐sensitive fluorochrome, Indo‐1. We found that elevation of [Ca2+]e failed to induce a rise of [Ca2+]i in quail osteoclasts, whilst causing an elevation of [Ca2+]i in rat osteoclasts. The calcium ionophore, ionomycin, led to a sustained elevation of [Ca2+]i in both cell types. These findings suggest that osteoclasts isolated from egg‐laying quail do not possess the calcium sensor or 'receptor' that appears to be vital for the survival and function of rat osteoclasts.