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Variations in [3H]ouabain binding of porcine skeletal muscle associated with feeding
Author(s) -
Dauncey MJ,
Burton KA,
Morovat A
Publication year - 1991
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1113/expphysiol.1991.sp003558
Subject(s) - ouabain , endocrinology , medicine , skeletal muscle , chemistry , biology , meal , zoology , anatomy , sodium , organic chemistry
The maximal ouabain binding capacity (Bmax) of longissimus dorsi muscle has been determined over a 24 h period in young pigs living at 12 degrees C. Animals were fed once a day and Bmax was estimated at 4, 8, 12 or 24 h after a large meal. Mean values of Bmax +/‐ S.E.M. (pmol ouabain/g wet wt muscle) of 548 +/‐ 78 and 556 +/‐ 57 at 4 and 24 h after feeding were significantly greater than those of 383 +/‐ 24 and 393 +/‐ 30 at 8 and 12 h after feeding (P less than 0.02). The extent to which these differences represent apparent or real changes in numbers of ouabain binding sites and, hence, in Na+,K(+)‐ATPase concentration is discussed.