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Arginine vasopressin stimulation of chloride secretion in Madin‐Darby canine renal epithelial layers
Author(s) -
Simmons NL,
Brown CD
Publication year - 1991
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1113/expphysiol.1991.sp003512
Subject(s) - vasopressin , medicine , endocrinology , forskolin , bumetanide , arginine , stimulation , secretion , chemistry , secretagogue , ion transporter , biology , biochemistry , amino acid , membrane
Arginine vasopressin (AVP) at a concentration of 0.1 microM stimulates an inward short‐circuit current (SCC) in voltage‐clamped Madin‐Darby canine kidney epithelial layers with a mean peak of 3.1 +/‐ 0.8 microA cm‐2 (n = 6). The response to AVP is markedly attenuated when inward SCC is stimulated with forskolin or prostaglandin E1, in Cl(‐)‐free media and by the Cl(‐)‐transport inhibitors, bumetanide and 5‐nitro‐2‐(3‐phenylpropylamino)‐benzoic acid, consistent with an AVP‐stimulated basal to apical Cl‐ secretion. Endogenous release of prostaglandins may contribute to AVP stimulation of Cl‐ secretion.