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Cumulative dose‐response curves for bethanechol‐induced electrogenic secretion in rat jejunum in vitro: is tachyphylaxis a significant factor?
Author(s) -
Przyborski SA,
Levin RJ,
Young A
Publication year - 1991
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1113/expphysiol.1991.sp003498
Subject(s) - tachyphylaxis , bethanechol , jejunum , medicine , secretagogue , endocrinology , chemistry , secretion , receptor , muscarinic acetylcholine receptor
Electrogenic secretion was monitored as the short‐circuit current (Isc) by an automatic voltage clamp across the isolated rat jejunum incubated in vitro. Responses to consecutive additions of bethanechol (1 mM) to the serosal surface showed tachyphylaxis. Addition of prostaglandin E2, however, after a single dose of 1 mM‐bethanechol, induced an undiminished Isc response indicating that the tachyphylaxis was homologous. Dose‐response curves for serosal applications of bethanechol obtained by serial‐cumulative addition did not show tachyphylaxis when compared with those from a non‐cumulative technique. The method of automatically recording the intestinal Isc with serial‐cumulative addition of a serosal secretagogue gives dose‐response curves free from tachyphylaxis.