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EXTRACELLULAR MAGNESIUM REGULATES ACETYL‐CHOLINE‐EVOKED AMYLASE SECRETION AND CALCIUM MOBILIZATION IN RAT PANCREATIC ACINAR CELLS
Author(s) -
Lennard Roger,
Francis Leo P.,
Singh Jaipaul
Publication year - 1989
Publication title -
quarterly journal of experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0144-8757
DOI - 10.1113/expphysiol.1989.sp003326
Subject(s) - extracellular , medicine , amylase , endocrinology , cytosol , chemistry , magnesium , calcium , secretion , acetylcholine , intracellular , pancreas , mobilization , choline , biology , enzyme , biochemistry , history , organic chemistry , archaeology
Application of ACh (10 −6 M) to the isolated rat pancreas elicited large increases in amylase secretion, radiolabelled 45 Ca influx and cytosolic free Ca 2+ levels in zero and normal (1·1 mM) extracellular magnesium (Mg 2+ ). Elevated (10 mM) Mg 2+ significantly ( P 〈 0·001) reduced the ACh‐induced amylase output and Ca 2+ mobilization. Similarly, ACh (10 −5 M) caused a transient increase in 45 Ca efflux from pre‐loaded pancreatic segments in normal Mg 2+ compared with the much reduced effect in elevated Mg 2+ . The results suggest that a modification in Mg 2+ influences ACh‐evoked amylase secretion by regulating Ca 2+ mobilization.